Fabrication of tissue engineered vein containing valve scaffolds
Liu, C.-z.; Lü, W.-m.; Yin, H.-h.; Zeng, C.-g.; Quan, D.-p.; Wang, W.-j.; Wang, S.-m.
Zhonghua Yi Xue Za Zhi 92(15): 1054-1057
2012
ISSN/ISBN: 0376-2491 PMID: 22781649 Document Number: 656412
To fabricate porous biodegradable tissue engineered vein containing valve scaffolds. Based on the self-made cast, the tissue engineered vein containing valve scaffolds was fabricated by injection molding plus thermally induced phase separation. Poly (lactic-co-glycolic acid) (PLGA, LA/GA mole ratio 75:25) was used as matrices. Morphological structures and biocompatibility of scaffolds were tested. Cell seeding on scaffold was performed and the mechanic characteristics of cellular constructs evaluated. The scaffold had an inner diameter of 9 mm with a wall thickness of 0.9 mm and the thickness of valves was (0.32 ± 0.04) mm. Scanning electron microscopic (SEM) micrographs showed regular ladder-like porous structures and the average pore size and porosity of scaffolds were 10 - 20 µm and 90%. The PLGA scaffolds were biocompatible. The cellular constructs were tested in vitro, and the valve leaflets were functionally capable of opening and closing when stimulated. Based on the self-made cast, the tissue engineered vein containing valve scaffolds can be fabricated by injection molding plus thermally induced phase separation. Further researches are warranted.