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WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 3rd ed.
Cambridge, England, Cambridge University Press, 1992. viii, 107 p.In 1992, WHO's special program of research, development and research training in human reproduction updated its laboratory manual for the examination of human semen and sperm-cervical mucus interaction because the field of andrology continues to progress quickly and there is heightened realization of the need for standardized measurement of all semen variables. This manual is designed to mainly serve the needs of researchers and clinicians in developing countries. Chapter 2 addresses laboratory procedures that are minimal essential steps for semen evaluation, optional procedures, and procedures needing additional evaluation, such as computer-aided sperm analysis used to measure sperm motion (i.e., research tools). The Shorr stain has replaced the Papanicolaou stain. Measurements of acid phosphatase and neutral alpha-glucosidase were added, while the measurement of adenosine triphosphatase was excluded. Few changes between the 2nd and 3rd editions occurred in Chapter 3. Chapters 4 and 5 are rather brief but discuss interlaboratory and technical standardization procedures. New appendices present safety guidelines and basic requirements for the andrology laboratory.