Title: Laboratory characterization of human T cell lymphotropic virus types 1 (HTLV-1) and 2 (HTLV-2) infections in blood donors from Sao Paulo, Brazil.

POPLINE Document Number: 128986

Author(s):

Segurado AA
Malaque CM
Sumita LM
Pannuti CS
Lal RB

Source citation:

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1997 Aug;57(2):142-8.

Abstract:

Blood donors in Brazil have recently begun to be screened for infection with HTLV types 1 and 2. Of 351,639 blood donations screened in Sao Paulo from January 1992 to October 1993, 1063 positive and 2238 indeterminate samples were identified based upon serologic confirmation using the 21e Western blot. Detailed serologic, molecular, and virologic analysis, based upon a laboratory diagnostic algorithm for the characterization of HTLV-1 and HTLV-2 infections, was conducted upon 50 seropositive or seroindeterminate blood donors. 2.3 Western blot serologic assays, which incorporate type-specific recombinant peptides, performed in 29 HTLV 1/2 positive and 21 HTLV 1/2 indeterminate donors with the 21e Western blot identified 25 as infected with HTLV-1, 4 with HTLV-2, 5 with untypeable HTLV 1/2, 15 as HTLV 1/2 indeterminate, and 1 as seronegative. Polymerase chain reaction (PCR) analysis using DNA amplification of proviral pol and tax sequences from peripheral blood mononuclear cells confirmed HTLV-1 and HTLV-2 infections in all 2.3 Western blot seropositive donors. Of the 5 serologically untypeable donors, 3 were found to be HTLV-1-positive, 1 HTLV-2-positive, and 1 negative by PCR. All seroindeterminate donors were also negative by PCR. HTLV-1 could be isolated in cocultures from 10 of 18 infected donors.

Keywords:

Brazil
Research Report
Urban Population
Screening
Prevalence
Blood Donors
HIV Infections
Developing Countries
South America, Eastern
South America
Latin America
Americas
Population Characteristics
Demographic Factors
Population
Examinations and Diagnoses
Measurement
Research Methodology
Blood Supply
Equipment and Supplies
Viral Diseases
Diseases