Title: [Determination of progesterone in saliva]

POPLINE Document Number: 081317

Author(s):

Herges H
Klingler W
Gethmann U
Knuppen R

Source citation:

ZENTRALBLATT FUR GYNAKOLOGIE, 1992;114(11):533-8.

Abstract:

The progesterone derivative, 11-alpha-glucuronide-iodine, tyramine (125I), was used for a radioimmunoassay analysis to study the possible correlation of progesterone concentration in saliva with progesterone level in serum. Female subjects had 2 ml of blood taken during the menstrual cycle in the morning after saliva samples were collected. Group A consisted of 1 women who used 2-3 citric acid crystals for inducing saliva production, while in Group B, consisting of 10 women, there was not such stimulation. Basal temperature was also measured. In Group A 1 of the women was taking a hormonal contraceptive, 8 women had regular cycles (24 an 20 days), and 2 had irregular cycles. In Group B 1 woman was also using contraceptives. 2 male subjects also participated in the saliva tests daily for 4 weeks. In precision measurements the intra-assay variation coefficient range within a series was 8.4% (lower concentration area) and 3.9% (higher concentration area). The sensitivity of the assay was 3.12 pg/ml in a volume of .4 ml (p 0.05). The 11 menstrual cycles of Group A consisted of 5 normal cycles, 5 with luteal insufficiency, and 1 under hormonal therapy. The 5 normal cycles showed a biphasic course of progesterone value in the saliva, whereby in the 2nd half of the cycle the values averaged about 100 pg/ml over the values of the follicular phase, which were 100 pg/ml compared to 200 pg/ml in the luteal phase. The progesterone values in the saliva of patients with corpus luteum insufficiency and the 1 under hormonal treatment did not show periodicity: the values ranged between 75 and 150 pg/ml. In Group B only 4 cycles had a biphasic course of progesterone in the saliva, in another 2 it was doubtful, and in the remaining 4 it did not show any periodicity. The progesterone values of the 2 males averaged from 80 to 120 pg/ml. The daily changes of saliva progesterone levels in 5 subjects indicated individual scatterings of 25 to 100 pg/ml from the average values. The progesterone profile of 3 subjects showed similar abrupt changes in 3 measurements but only a 20 pg/ml deviation from the average.

Keywords:

Germany
Progesterone
Measurement
Hematologic Tests
Infertility
Menstrual Cycle
Laboratory Procedures
Europe, Central
Europe
Developed Countries
Progestational Hormones
Hormones
Endocrine System
Physiology
Biology
Research Methodology
Laboratory Examinations and Diagnoses
Examinations and Diagnoses
Reproduction
Menstruation