POPLINE Document Number: 017013
Author(s):
Witkin SS
Brandslund I
Svehag SE
Linnet L
Peter N
Moller H
Source citation:
Journal of Clinical and Laboratory Immunology, 1983 Apr;10(4):193-7.
Abstract:
Sera from 46 vasectomized men for 5 years and from 46 age-matched nonvasectomized men had previously been analyzed for circulating immune complexes (CICs) by 4 assays. 4 additional CIC assays have now been performed on these sera: 3 Raji cell enzyme-linked immunosorbent assays (ELISAs) using alkaline phosphatase-conjugated antihuman IgG, IgA, or C3 and the bull sperm ELISA. No significant differences in CIC levels were detected between the 2 groups using any of the assays. Results for 2 different sera obtained from 16 men 4 1/2 months apart correlated significantly for 6 of 7 CIC assays evaluated in this way. In the bull sperm and Raji cell ELISAS, utilizing anti-IgG in the detection layer, the vasectomized men with sperm agglutinating antibodies were found to have significantly higher CIC levels than those without sperm agglutinants. No association was found between the presence of sperm protamine antibody and levels of CICs. Since the vasectomized and control groups did not differ with respect to levels of CICs, immunoglobulins, or the complement C3 split product Cd3, all 92 samples were combined into 1 group for further analysis. Serum IgG levels significantly correlated with the CIC levels in 4 of 5 CIC assays involving binding of IgG; IgA levels correlated with CIC determinations in the Raji-IgA assay, while plasma C3d levels correlated with the Raji-C3, the Raji-IgA, and the Cl1-protein A binding assays. IgM levels did not correlate with activity in any assay. Finally, the degree of correlation between all 8 CIC assays was determined, and significant positive correlations between assays were found in 10 of 28 comparisons. (author's)
Keywords:
VasectomyIndex page
Male Sterilization
Male Urologic Surgery
Sterilization, Sexual
Autoimmune Response
Antibodies
Analysis
Laboratory Procedures
Family Planning
Urogenital Surgery
Surgery
Treatment
Immunologic Factors
Immunity
Immune System
Physiology
Biology
Research Methodology
Laboratory Examinations and Diagnoses
Examinations and Diagnoses